Album Review: At War With The Silverfish by Laura Jane Grace on Polyvinyl Record Co. and Big Scary Monsters (by Ilse R. Smit)

2022.01.26 08:52 ColinsPunkRockWorld Album Review: At War With The Silverfish by Laura Jane Grace on Polyvinyl Record Co. and Big Scary Monsters (by Ilse R. Smit)

Album Review: At War With The Silverfish by Laura Jane Grace on Polyvinyl Record Co. and Big Scary Monsters (by Ilse R. Smit) submitted by ColinsPunkRockWorld to Punk_Rock [link] [comments]


2022.01.26 08:52 rfwoolf What comes before the Paranoid-Schizoid position? Would it be psychosis? (Image attached)

What comes before the Paranoid-Schizoid position? Would it be psychosis? (Image attached)
https://preview.redd.it/vx1rlz2kt0e81.jpg?width=561&format=pjpg&auto=webp&s=bc8b238e8a9669eb4ba58a8937a3f0c0265bf8a9
submitted by rfwoolf to psychoanalysis [link] [comments]


2022.01.26 08:52 ShutRopes Laptop suggestion


submitted by ShutRopes to SuggestALaptop [link] [comments]


2022.01.26 08:52 FrontpageWatch2020 [#6|+11192|197] Why they always have to kiss [r/Unexpected]

submitted by FrontpageWatch2020 to undelete [link] [comments]


2022.01.26 08:52 BenzDriverS SARS-Cov2 "Isolation" - Take a look at how it is done.

SARS-Cov2 "Isolation"

Vero CCL-81 cells were used for isolation and initial passage. Vero E6,Vero CCL-81, HUH 7.0, 293T, A549, and EFKB3 cells were cultured inDulbecco’s minimal essential medium (DMEM) supplemented with heatinactivated fetal bovine serum(5 or 10%) and antibiotic/antimyotic(GIBCO). Both NP an OP swabs were used for virus isolation. For theisolation, limiting dilution, and passage 1 of the virus, 50 μl serumfree DMEM was pipetted into columns 2–12 of a 96-well tissue cultureplate. One-hundred μl clinical specimens were pipetted into column 1,and then serially diluted 2-fold across the plate. Vero cells weretrypsinized and resuspended in DMEM + 10% FBS + 2XPenicillin-Streptomycin + 2X antibiotic − antimycotic + 2 X amphotericinB at 2.5 × 105 cells / ml. One hundred μl of cell suspensionwere added directly to the clinical specimen dilutions and mixed gentlyby pipetting. The inoculated cultures were grown in a humidified 37°Cincubator with 5% CO2 and observed for cytopathic effect (CPE) daily.Standard plaque assays were used for SARS-CoV-2 based on both SARS-CoVand MERS-CoV protocols (19, 20).
Fetal Bovine Serum RNA Interferes with the Cell Culture derived Extracellular RNA
Fetal Bovine Serum - a cell culture dilemma
Amphotericin B nephrotoxicity
How is this "isolation"?
submitted by BenzDriverS to actualScience [link] [comments]


2022.01.26 08:52 billbones17 If your life was to have a soundtrack, which songs would feature?

submitted by billbones17 to AskReddit [link] [comments]


2022.01.26 08:52 FrontpageWatch2020 [#3|+19896|158] If you're giving them then I'll give them too!! [r/youseeingthisshit]

submitted by FrontpageWatch2020 to undelete [link] [comments]


2022.01.26 08:52 allay What is going on with this fern? (Nephrolepis Exaltata)

What is going on with this fern? (Nephrolepis Exaltata) submitted by allay to plantclinic [link] [comments]


2022.01.26 08:52 Ihaveseenthings---- 19m. A year ago my ex left me and all of my friends either tried to sleep with her or cut me off, i hit rock bottom, I know I didn’t deserve that, but I’m glad it happened, I’m not happy and I feel lonely a lot, but I learned from those events and the only way to go is up!

19m. A year ago my ex left me and all of my friends either tried to sleep with her or cut me off, i hit rock bottom, I know I didn’t deserve that, but I’m glad it happened, I’m not happy and I feel lonely a lot, but I learned from those events and the only way to go is up! submitted by Ihaveseenthings---- to toastme [link] [comments]


2022.01.26 08:52 Visible-Reading-3334 Which one is harder to form? SPQR or mongol empire?

me and my friend keep fighting about which one is harder to form overall so we just want to get this over with and ask what most of the community thinks
submitted by Visible-Reading-3334 to eu4 [link] [comments]


2022.01.26 08:52 biggiesmallz232 Cheekies!!!!!

Cheekies!!!!! submitted by biggiesmallz232 to ChriseanRock [link] [comments]


2022.01.26 08:52 AspectLeft5556 Jemand Lust auf Rp? Dm…

submitted by AspectLeft5556 to Sinamarie [link] [comments]


2022.01.26 08:52 NewsElfForEnterprise TV's Antihero Era Is Over. Welcome to the Golden Age of Hope.

TV's Antihero Era Is Over. Welcome to the Golden Age of Hope. submitted by NewsElfForEnterprise to News_Entertainment [link] [comments]


2022.01.26 08:52 Eggplantingzz Can i buy and sell NFTs with ETH under the age of 18

I'm fairly new to the whole NFT thing, so forgive me if i say anything dumb. I've recently become familiar with the concept of the "wallet". I set up my wallet in metamask and tried to buy a small amount of ETH. However when i set up my date of birth, metamask wouldn't accept it. I'm 16 turning 17 this year. Is it possible for me to trade ETH and buy NFTs. Or do i have to be over a certain age?
submitted by Eggplantingzz to NFT [link] [comments]


2022.01.26 08:52 Hazen1223 Discussion About Full Band Drops

Hello! I currently have just heard the song Hurricane by I Prevail ( I know I know). I am blown away by this song and was thinking to myself with the first drop of the full band playing “is this the best full band drop (or if I’m saying that wrong please correct me) ever? I would like to use this thread to discuss! Also please be nice to each other and their opinions. I look forward to hearing what is YOUR favorite drop is!
submitted by Hazen1223 to Metalcore [link] [comments]


2022.01.26 08:52 NFT_DigitalArt FREE NFT GIVEAWAY!🎁 JOIN DISCORD & DROP YOUR ADDRESS! 🔥 [X-post from /r/opensea]

FREE NFT GIVEAWAY!🎁 JOIN DISCORD & DROP YOUR ADDRESS! 🔥 [X-post from /opensea] submitted by NFT_DigitalArt to DigitalItems [link] [comments]


2022.01.26 08:52 JonASMRscripts [F4M] Caring for the cursed Wolf [fantasy setting] [care] [rambling] [story telling] [lonely maiden]

Please read up on my FAQ when first working with my scripts, thank you.
SCRIPT HERE: https://docs.google.com/document/d/e/2PACX-1vQle08B9EI55eKK1R6qhjXFGSdcO4flgrWQJ87Vw54PcyEINYO3J7eKN7sYoLonrxasw8SRl5syhyLS/pub
Summary: The [Listener] as a cursed wolf finds himself on the porch of a decrepit hut where the a lonely maiden cares for him, all the while telling her tale of how she ended up in this place, of a dark witch and her evil machinations.
This one is a personal favorite.
Did you find that of interest? Feel free to look through my body of work.
As stated in my FAQ, feel free to monetize, but do not paywall. Post on YouTube, SoundCloud, whathaveyou... All that is ever truly required as recompense is credit. Just something along the lines of: "Script by: JonASMRscripts" , no link required (or even wanted, since some sites can be quite fickle in that regard).
submitted by JonASMRscripts to ASMRScriptHaven [link] [comments]


2022.01.26 08:52 alexanderleroy01 Aluminum Doors vs Wooden Doors- Which one requires frequent repairs?

Aluminum Doors vs Wooden Doors- Which one requires frequent repairs? submitted by alexanderleroy01 to promote [link] [comments]


2022.01.26 08:52 UrartuQueen Arayik Harutunyan, the Chief of Staff to the Prime Minister, will be the new president of the Republic of Armenia - based on Pashinyan’s decision. (Unconfirmed)

Arayik Harutunyan, the Chief of Staff to the Prime Minister, will be the new president of the Republic of Armenia - based on Pashinyan’s decision. (Unconfirmed) submitted by UrartuQueen to armenia [link] [comments]


2022.01.26 08:52 ArshiyaEsf ‼️EXCLUSIVE DAO COMMUNITY‼️

This is a brand new DAO Project Im currently working on. We are handing out OG Roles to the next 100 people that join.
This project will give you access to the peak of the pyramid in which all royalties from future projects and secondary markets go into the pockets of our 130 holders. We plan on releasing a bot, a P2E Game, a Ponzi project and much much more, with 100% of the profits going to our 130 holders.
Exclusive DAO community is already set up for those with OG roles in which we call upcoming NFT projects and have an Alpha for daily mints, we also share research between our members allowing you to come up with the best decision. We also are in talks with partnerships for WL for other projects and NFT drops from other projects. We also have beginner roles for people who need help getting started with NFT’s so we can help any newcomers.
This project is very unknown, in-fact this is the first shill post I’ve made and will most likely remain as the only one I will ever make. This is because we want to keep our community as exclusive as possible, and in order to do this we will soon turn off invites and joining.
https://discord.gg/85eM9A4Q
submitted by ArshiyaEsf to NFTMarketplace [link] [comments]


2022.01.26 08:52 ThatsSo-Fetch Scanty/scalpy locs

Scanty/scalpy locs submitted by ThatsSo-Fetch to Dreadlocks [link] [comments]


2022.01.26 08:52 proedross Έρχεται στο Disney+ στην Ελλάδα- Νέος streaming προορισμός (το καλοκαίρι)

Έρχεται στο Disney+ στην Ελλάδα- Νέος streaming προορισμός (το καλοκαίρι) submitted by proedross to greece [link] [comments]


2022.01.26 08:52 Kitchen-Substance707 as an elise main, I'm sad she doesn't have more skins. so I decided to start to work on a new skin for her:D I only have a spider form concept ready but I think it has potential! im planning to work on a prestige version as well^^ would like to know what elises around the world think about this:)

as an elise main, I'm sad she doesn't have more skins. so I decided to start to work on a new skin for her:D I only have a spider form concept ready but I think it has potential! im planning to work on a prestige version as well^^ would like to know what elises around the world think about this:) submitted by Kitchen-Substance707 to Elisemains [link] [comments]


2022.01.26 08:52 Sleeper2020 Has anyones memory loss / brain fog returned after a loss? If so, how long after?

submitted by Sleeper2020 to GriefSupport [link] [comments]


2022.01.26 08:52 giacoboh88 What kind of flower is this? (Italy)

What kind of flower is this? (Italy) submitted by giacoboh88 to Wildflowers [link] [comments]


http://aps-education.ru